Microwave Immunohistochemistry on Mohs Cryostat Sections
نویسندگان
چکیده
منابع مشابه
Use of microwave oven improves morphology and staining of cryostat sections.
The quality of microscopic image of cryostat sections that had been subjected to microwave assisted fixation was compared with that resulting from conventional air drying of the sections. The role of microwaves in producing rapid special stains on cryostat sections was also assessed. Methods used permitted stains such as periodic acid Schiff, alcian blue, Gordon and Sweets's reticulin, Masson F...
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A recently developed procedure, that has been shown to be suitable for detailed immunohistological analysis, has been used to prepare cryostat sections of bone marrow to investigate whether enzyme-histochemical techniques are also feasible on such material. A selected group of enzymes, some of which are inhibited or destroyed in paraffin- or plastic-embedded samples, have been demonstrated. The...
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A bone cryostat is described with which cold sections of unfixed, undemineralized calcified tissue can be prepared. The bone cryostat is suitable for the preparation of the sections required for the histochemical and immunofluorescent study of bone, for electron microprobe analysis, and for bone and joint autoradiography with soluble compounds.
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To localize factors that guide axons reinnervating skeletal muscle, we cultured ciliary ganglion neurons on cryostat sections of innervated and denervated adult muscle. Neurons extended neurites on sections of muscle (and several other tissues), generally in close apposition to sectioned cell surfaces. Average neurite length was greater on sections of denervated than on sections of innervated m...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 2000
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s155192950005464x